Ultrasensitive Bioanalytical Assays for Cytotoxic Drugs: Focus on Locally Administered Anti-Cancer Agents

摘要

Local administration routes have been investigated to reduce the systemic toxicity and to increase the local efficacyof cytotoxic drugs. Some examples of local administration strategies are cutaneous, intraperitoneal, intrathecal andintravesical chemotherapy. When administered locally, high local drug concentrations can be achieved with increased localefficacy and, conditionally that only small amounts of drug are absorbed into the bloodstream, low systemic toxicity.Our main purpose is to make an inventory and to comment on the availability of ultrasensitive bioanalytical assays thatcould determine traces of the drugs that may have passed into the bloodstream, e.g. after local application, and which maylead to the systemic toxicity. We conclude that in the last years, multiple ultrasensitive assays have been designed capableto quantitatively determine very low levels of cytotoxic agents e.g. systemically reached after local administration. Mostmethods are based on the hyphenated liquid chromatography with tandem mass spectrometric detection.