Identification of Alkaloid Compound and Antioxidant Activity of Rafflesiacantleyi and its Host, Tetrastigma tuberculatum

摘要

Alkaloid compounds in Rafflesia cantleyi and host, Tetrastigma tuberculatum were identified using thephytochemical screening method and HPLC technique. The results showed positive test for the host, T. tuberculatumwhich indicates the presence of alkaloid whereas the screening test for R. cantleyi showed that alkaloid is absenced. Bothextract were then subjected to HPLC for alkaloid identification and caffeine was used as standard. The spectroscopic datafrom HPLC revealed the presence of caffeine in both methanol extracts. The methanol extract of both samples were alsotested for antioxidant activity by using DPPH free radical scavenging activity. Result showed that the radical scavengingactivity of R. cantleyi and T. tuberculatum are comparable to the standard reference, BHT but higher than the standardalpha tocopherol.