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Isolation of Chlamydia psittaci from Domestic Cats with Oculonasal Discharge in Japan

机译:日本眼耳分泌物从家猫中分离出鹦鹉热衣原体

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References(13) Cited-By(4) Eight strains of Chlamydia psittaci were isolated in Japan from the nasal and conjunctival swabs of six household cats using the L929 cell line of mouse fibroblast origin. The isolates were identified as C. psittaci on the basis of the formation of characteristic inclusion bodies in the cell culture detected by Giemsa stain and immunofluorescence. Comparison of nucleotide sequences of the ompA gene amplified from the three isolates with the published sequence of feline FEPN strain of C. psittaci showed almost 100% homology.
机译:参考文献(13)引用了(4)在日本,使用小鼠成纤维细胞来源的L929细胞系从六只家猫的鼻和结膜拭子中分离出八株鹦鹉热衣原体。根据通过吉姆萨染色和免疫荧光检测到的细胞培养物中特征性包涵体的形成,将分离物鉴定为鹦鹉热衣原体。从三个分离物中扩增的ompA基因的核苷酸序列与鹦鹉热衣原体的猫FEPN菌株的公开序列进行比较显示出几乎100%的同源性。

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