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Cytomorphology Of Hydatid Disease

机译:包虫病的细胞形态

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We present a case of hydatid disease and conclude that in a patient with a presumptive diagnosis of cystic hydatid disease, FNA is the primary method of confirming hydatid disease. Case Report A 40-year-old female complained of dull pain abdomen for the last 3 months. On per abdominal examination, no mass was palpable. Her routine hematological and biochemical investigations were within normal limits. Ultrasound abdomen revealed a 6x3.5 cm, unilocular hyperechoic lesion in right lobe of the liver. A radiological diagnosis of abscess liver was made. Hydatid serology was negative. Ultrasound guided fine needle aspiration (FNA) was done with 22gauge lumbar puncture needle and 110ml turbid fluid was aspirated. No untoward allergic reaction was observed following FNA .The fluid was centrifuged and smears were prepared from the sediment. The smears were stained by May-Grünwald Giemsa (MGG), haematoxylin & eosin (H&E) and Masson’s trichrome (MT) stains. Microscopic examination revealed numerous hooklets, laminated membrane and scolices. No inflammatory cells were seen. The laminated membrane appeared as fragments of acellular material with delicate parallel striations staining deep blue (Figure1). Many scolices which were round to oval structures about 100μm in diameter with one or two rows of characteristic radially arranged hooklets were seen (Figure 2). The hooklets were about 20-40um in size, semi-translucent, refractile, triangular or sickle-shaped with an inner semi-translucent core of the same shape. The hooklets were better highlighted by MT stain (Figure 2; Inset). Some scolices showed flame cells and were without hooklets (Figure 3). With these cytological findings, a diagnosis of hydatid cyst was made.
机译:我们提出一例hy虫病,并得出结论,在假定诊断为囊状hy虫病的患者中,FNA是确认hy虫病的主要方法。病例报告一名40岁的女性抱怨最近3个月腹部腹部疼痛。每次腹部检查均未触及肿块。她的常规血液学和生化检查在正常范围内。腹部超声显示肝脏右叶有一个6x3.5 cm的单眼高回声病变。对脓肿肝脏进行了放射学诊断。包虫血清学阴性。用22号腰椎穿刺针进行超声引导的细针穿刺(FNA),并抽吸110ml浑浊的液体。 FNA后未观察到不良的过敏反应。将液体离心并从沉积物中制备涂片。涂片用May-GrünwaldGiemsa(MGG),苏木精和曙红(H&E)和Masson三色(MT)染色。显微镜检查发现无数钩,夹层膜和粘液。未见炎性细胞。层压膜表现为无细胞材料的碎片,具有细腻的平行条纹,染成深蓝色(图1)。可以看到许多圆形到椭圆形结构,直径为100μm且具有一排或两排特征性径向排列的钩状结构的纤维板(图2)。小钩的大小约为20-40um,为半透明,可折射,三角形或镰刀形,内部半透明芯相同。 MT染色可更好地突出显示小钩(图2;插图)。一些黏膜显示出火焰细胞,没有钩状细胞(图3)。有了这些细胞学发现,就可以诊断出包虫囊肿。

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