Azospirillum palatum sp. nov., isolated from forest soil in Zhejiang province, China

摘要

A Gram-negative bacterium designated ww 10T was isolated by plating dilutions from forest soil in Zhejiang province, China. Strain ww 10T was investigated by polyphasic taxonomic study including phenotypic and phylogenetic analysis. Cells of ww 10T were Gram-negative, strictly aerobic, motile with peritrichous flagella and rod-shaped. The strain grew optimally at 30-37ºC and pH 6.0-8.0. The major fatty acids were C18:1ω7C, cyclo-C19:0 ω8C and C16:0. The respiratory quinones contained a large amount of Q-10, a moderate component of Q-9 and a minor of Q-8. The G+C content of genomic DNA was about 67.3 mol%. Phylogenetic analysis revealed that strain ww 10T belongs to the phyletic cluster of genus Azospirillum and displayed 16S rRNA gene sequence similarity lower than 96.3% to the four closest described species of the genera Azospirillum and Roseomonas. Results of polyphasic taxonomic analysis showed that strain ww 10T represents a novel species in the genus Azospirillum, for which the name Azospirillum palatum sp. nov., is proposed. The type strain is ww 10T (=LMG 24444T=KCTC 13200T=CCTCC AB 207189T).