Biomolecular Aspect of Apoptosis Pathway: Caspase-8 and Caspase-9 on Polifenol Exposure of Phaleria macrocarpa (Scheff.) Boerl. on Mice Balb/c

摘要

Objective: The Polyphenol compound of Phaleria macrocarpa has potential as an anticancer agent, and it has shown to inhibit lung carcinogenesis. The objective of this study was to elucidate the role of polyphenol induced apoptosis via caspase-8 and caspase-9. Methods: Balb/c mice were randomly divided into 2 groups: Group 1: control group with administration of sterile aquades and group 2: treatment group with administration of 50 mg polyphenols. The development of lung tumors was confirmed by observing post-operation tissue at week 8, 17 and 26. The expression of caspase-8 and caspase-9 were assayed. Data was analyzed using Kruskal-Wallis, Mann-Whitney, one way ANOVA, Post hoc LSD test, with a significance level of p < (0.05). Results: Administration of 50 mg polyphenols from P. macrocarpa extract, showed inhibition of lung carcinogenesis through increased expression of caspase-8 and caspase-9 in the treatment group at week 8, 17 and 26 (p = 0.000). Conclusion: The administration of poliphenol from P. macrocarpa extract was shown to effectively inhibited lung carcinogenesis through increased of caspase-8 and caspase-9 in mice strain Balb/c.