ANTIBACTERIAL COMPOUNDS PRESENT ON n-HEXANE EXTRACT OF TEMPUYUNG LEAVES ( Sonchus arvensis L.)

摘要

Isolation of antibacterial compounds from n-hexane extract of tempuyung leaf (Sonchus Arvensis L.) has been carried out. n-hexane extract (9,15 g) showed? antibacterial inhibition zone of 9 mm on Staphylococcus aureus and 8 mm on Escherichia coli at concentration of 1000 ppm.? Fractionation of n-hexane extract using column chromatography resulted on 5 fractions (F_(1), F_(2), F_(3), F_(4), and F_(5)). Antibacterial test of the fractions toward Staphylococcus aureus and Escherichia coli showed that fraction F_(2) and F_(4) positive toward Staphylococcus aureus and Escherichia coli. Fraction F_(3 )showed just positive toward Escherichia coli while fraction F_(5) just positive toward Staphylococcus aureus. Further separation using preparative thin layer chromatography (TLC) technical resulted in 3 fractions (F_(4.1), F_(4.2 )and F_(4.3 )fractions), in which F_(4.1 )fraction indicates the most active inhibition growth test again bacterial with diametric inhibition zone of 9 mm towards Staphylococcus aureus and 11 mm for Escherichia coli within concentration of 100 ppm.?? Gas Chromatography–Mass Spectroscopy (GC-MS) was employed in order to identity the F_(4.1) fraction. From WILEY7 database, it was identified ?that isolate are mixture? of 2? compound components namely bis (2-ethylhexyl) ester and possibility of new compound included triterpenoid group? with molecule formula C_(32)H_(66)O_(6).