Comparison of antibacterial activity inhibitory of black cumin (Nigella sativa) oil, Cresophene?, and Calcium hydroxide

摘要

ABSTRACT Introduction: Black cumin (Nigella sativa) oil is a natural antibacterial product containing thymoquinone. Thymoquinone is a powerful antibacterial substance towards gram-positive bacteria. The research objective was to compare the inhibitory effects of black cumin oil, Cresophene? and Ca(OH) 2 towards the growth of Staphylococcus aureus. Methods: Experimental laboratory by taking Staphylococcus aureus from deciduous teeth pulp necrosis that has been isolated in Brain-Heart Infusion (BHI) medium. Inhibitory of black cumin oil, Cresophene? and Ca(OH) 2 were measured by making three different 6 mm diameter wells contained each substances. Data then analyzed by two-way ANOVA using statistical analysis program. Results: Cresophene? had the largest inhibitory zone with the average zone was 32 ± 0.05mm and stable from the 1 st day until the 4 th day then decreased on the 5 th day and remain stable until the 7 th day. Ca(OH) 2 had average inhibitory zone of 15.9 ± 0:10 mm and remain stable from the 1 st day until the 7 th day. Black cumin oil had average inhibitory zone of ± 7.9 ± 0.2 mm and remain stable from the 1 st day until the 7 th day. Conclusion: The inhibitory zone towards Staphylococcus aureus isolated from deciduous teeth pulp necrosis consecutively was Cresophene?, Ca(OH) 2 and black cumin oil. Keywords : Staphylococus aureus, Black cumin oil, Cresophene? , Ca(OH) 2 , Inhibitory zone ?.