WRKY23 is a component of the transcriptional network mediating auxin feedback on PIN polarity

摘要

Auxin is unique among plant hormones due to its directional transport that is mediated by the polarly distributed PIN auxin transporters at the plasma membrane. The canalization hypothesis proposes that the auxin feedback on its polar flow is a crucial, plant-specific mechanism mediating multiple self-organizing developmental processes. Here, we used the auxin effect on the PIN polar localization in Arabidopsis thaliana roots as a proxy for the auxin feedback on the PIN polarity during canalization. We performed microarray experiments to find regulators of this process that act downstream of auxin. We identified genes that were transcriptionally regulated by auxin in an AXR3/IAA17- and ARF7/ARF19-dependent manner. Besides the known components of the PIN polarity, such as PID and PIP5K kinases, a number of potential new regulators were detected, among which the WRKY23 transcription factor, which was characterized in more detail. Gain- and loss-of-function mutants confirmed a role for WRKY23 in mediating the auxin effect on the PIN polarity. Accordingly, processes requiring auxin-mediated PIN polarity rearrangements, such as vascular tissue development during leaf venation, showed a higher WRKY23 expression and required the WRKY23 activity. Our results provide initial insights into the auxin transcriptional network acting upstream of PIN polarization and, potentially, canalization-mediated plant development. Author summary The plant hormone auxin belongs to the major plant-specific developmental regulators. It mediates or modifies almost all aspects of plant life. One of the fascinating features of the auxin action is its directional movement between cells, whose direction can be regulated by auxin signaling itself. This plant-specific feedback regulation has been proposed decades ago and allows for the self-organizing formation of distinct auxin channels shown to be crucial for processes, such as the regular pattern formation of leaf venation, organ formation, and regeneration of plant tissues. Despite the prominent importance of this so called auxin canalization process, the insight into the underlying molecular mechanism is very limited. Here, we identified a number of genes that are transcriptionally regulated and act downstream of the auxin signaling to mediate the auxin feedback on the polarized auxin transport. One of them is the WRKY23 transcription factor that has previously been unsuspected to play a role in this process. Our work provides the first insights into the transcriptional regulation of the auxin canalization and opens multiple avenues to further study this crucial process.