Efficient Plant Regeneration from Shoot Apices of Pearl Millet (Pennisetum americanum (L.) Leeke)

摘要

An efficient and rapid regeneration protocol was developed from shoot tip explants of Pennisetum americanum (L.) Leeke variety WC-75. MS supplemented with low concentration of 2,4-D and Kn was most effective in producing embryogenic calli. Maximum regeneration potential of 40 shoots per calli were obtained when transferred to regeneration medium containing 1.0 mg/l Kn. Shoots developed were efficiently rooted within 15 days on the medium containing NAA. Over 90% of rooted plants were fertile after transfer to a net house.?Key words: Pearl millet, Efficient, plant regeneration, Shoot apices?D.O.I. 10.3329/ptcb.v20i1.5964?Plant Tissue Cult. & Biotech. 20(1): 47-53, 2010 (June)