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>Rapid Development of PLBs Derived from Immature Seeds and Mass Multiplication of Vanda tessellata (Roxb.) Hook. Ex G. Don - A Threatened Orchid species in Bangladesh
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Rapid Development of PLBs Derived from Immature Seeds and Mass Multiplication of Vanda tessellata (Roxb.) Hook. Ex G. Don - A Threatened Orchid species in Bangladesh
A rapid and an efficient protocol for germination of immature seeds of Vanda tessellata, PLBs development and plant regeneration system have been developed. The results indicate that immature capsules performed better in terms of germination (100.0%) and development of PLBs (90.0%). Around 45 - 60 days old PLBs were transferred to half strength of MS supplemented with different concentrations of auxins and cytokinins. The highest weight of PLBs (5.53 g), the best plant regeneration (16.50) and shoot length (3.25 cm) were recorded when the medium was supplemented with BAP (1.0 mg/l) and NAA (1.5 mg/l). The highest number (5.0) and length (3.22 cm) of roots, the maximum number of plantlets (3.75) were obtained in the medium supplemented with 1.5 mg/l BAP and 1.0 mg/l IAA. The maximum numbers of leaves (3.33) were recorded in the medium fortified with 1.5 mg/l BAP + 1.00 mg/l IAA; the highest length of leaves (2.59 cm) was observed in the medium combining with of BAP and IAA (2.0 mg/l). Well shooted - and rooted plants were acclimated in pots that contained two types of compost. Here, type-II showed better performance on plant development.Plant Tissue Cult. & Biotech. 25(2): 181-191, 2015 (December)
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机译:已经开发了一种快速有效的方法来繁育特斯万代未成熟种子,PLBs发育和植物再生系统。结果表明,未成熟的胶囊在发芽(100.0%)和PLBs(90.0%)方面表现更好。大约45至60天大的PLB被转移至MS的一半强度,并补充了不同浓度的生长素和细胞分裂素。当向培养基中补充BAP(1.0 mg / l)和NAA(1.5 mg / l)时,记录到最高的PLB重量(5.53 g),最佳的植物再生(16.50)和枝条长度(3.25 cm)。在补充有1.5 mg / l BAP和1.0 mg / l IAA的培养基中,获得了最高的根数(5.0)和根长(3.22 cm),最大的幼苗数(3.75)。在以1.5 mg / l BAP + 1.00 mg / l IAA强化的培养基中记录了最大叶数(3.33);在与BAP和IAA(2.0 mg / l)混合的培养基中观察到最大的叶子长度(2.59 cm)。芽好-使生根的植物在装有两种堆肥的盆中适应。在这里,II型在植物发育方面表现出更好的表现。和生物技术。 25(2):181-191,2015(12月)
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