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首页> 外文期刊>Pharmaciana >ISOLASI DAN AKTIVITAS LARVACIDA MINYAK ATSIRI AKAR (Curcuma mangga, Val.) TERHADAP LARVA Aedes Aegypti DAN PROFIL GC-MS NYA
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ISOLASI DAN AKTIVITAS LARVACIDA MINYAK ATSIRI AKAR (Curcuma mangga, Val.) TERHADAP LARVA Aedes Aegypti DAN PROFIL GC-MS NYA

机译:埃及伊蚊幼虫的幼虫根精油的分离和活性(姜黄芒果,Val。)

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Curcuma mangga, Val. has been recognized as a traditional drug since a long time ago. The active ingrediences of this plant especially the volatile oil contains monoterpenes and sesquiterpenes. This research was aimed to study about the larvacide’s activity of the volatile oil Curcuma mangga, Val. rhizome and also to analyze its chemical compounds using the GC-MS. The volatile oil was isolated from Curcuma mangga, Val. with steam and water distillation. The test method for larvicide was done by dissolving the substances into water and added by Tween 20 10% v/v to the the volatile oil. The concentration of the volatile oil of Curcuma mangga , Val. rhizome were 100 ppm, 150 ppm, 200 ppm, 250 ppm, 300 ppm, and 350 ppm. The concentration of positive control “abate” were 0,01 ppm; 0,025 ppm; 0,05 ppm; 0,1 ppm; 0,5 ppm while as the negative control was Tween 20 solution. The perception time of larvae’s mortality was 24 hours. The chemical compound of Curcuma mangga , Val. can be analysed by the GC-MS. Data of larvae’s mortality were used to estimate the values of LC50 with the probit analyzis method. This study showed that the volatile oil of Curcuma mangga, Val. have clear-brass colour, bitter taste, typically aromatic like mango, rendement equal to (1,23 ± 0,029)% v/b and refractive index 1,4881. The value of LC50 for the volatile oil of Curcuma mangga, Val. rhizome is (216,17 ±12,51) ppm while abate equal to (0,072 ± 0,024) ppm. This finding indicates that abate were more potent to larvae of Aedes aegypti. The GC-MS showes 30 peaks chromatogram and six peaks which indicates the possibility of alpha-pinene, camphene, beta-pinene, beta-myrcene, eukalyptole, ar-turmerone presence.
机译:姜黄,Val。早在很久以前就被公认为传统药物。该植物的活性成分,尤其是挥发油中含有单萜和倍半萜。这项研究旨在研究挥发油姜黄(Curcuma mangga,Val)的幼虫活性。根茎,并使用GC-MS分析其化学成分。挥发油是从Val。Curcuma mangga分离得到的。用蒸汽和水蒸馏。杀幼虫剂的试验方法是将物质溶解在水中,然后以吐温20 10%v / v的量添加到挥发油中。姜黄挥发油的浓度,Val。根茎为100 ppm,150 ppm,200 ppm,250 ppm,300 ppm和350 ppm。阳性对照“消融”的浓度为0.01 ppm; 0,025 ppm; 0.05 ppm; 0.1 ppm; 0.5 ppm,而吐温20溶液为阴性对照。幼虫死亡率的感知时间为24小时。姜黄的化学化合物,Val。可以通过GC-MS进行分析。幼虫死亡率的数据用于通过概率分析法估算LC50的值。这项研究表明,姜黄的挥发油,Val。具有清澈的黄铜色,苦涩的味道,通常像芒果一样芳香,凝结度等于(1,23±0,029)%v / b,折射率为1,4881。姜黄姜的挥发油的LC50值。根茎为(216,17±12,51)ppm,而消融度等于(0,072±0,024)ppm。这一发现表明,缓蚀剂对埃及伊蚊的幼虫更有效。 GC-MS显示30个色谱峰和6个峰,表明存在α-pine烯、,烯,β-pine烯,β-月桂烯,桉叶油,ar-turmerone的可能性。

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