PENETAPAN KADAR FENOLIK TOTAL EKSTRAK METANOL KELOPAK BUNGA ROSELLA MERAH (Hibiscus sabdariffa Linn) DENGAN VARIASI TEMPAT TUMBUH SECARA SPEKTROFOTOMETRI

摘要

The purpose of this research is to determine the total phenolic content of Hibiscus sabdariffa calyx in variations of growing area. Red Rosell calyxs were collected from Glagah, Kediri, and Samigaluh. Phenolic compounds of Hibiscus sabdariffa calyx were extracted using maceration method with methanol. Total phenolics content were determined using visible spectrophotometry method with Folin Ciocalteau reagent. The principle of this method is the formation of blue complex compound from phospomolybdate-phosphotungstate reduced by phenolic compound in the basic condition, which can be measured by spectrophotometry. Galic acid was used as comperator in this research. Total phenolic content in red calyx Glagah, Kediri and Samigaluh were respectively 1.40 g GAE/100 g extract, SD 0.06 (n=12), 1.41 g GAE/100 g extract, SD 0.07 (n=12) dan 2.12 g GAE/100 g extract, SD 0.05 (n=15). Based on this results it could be concluded that growing area affected total phenolic content in the methanol extract of red calyx Rosell.