HPTLC Method Development and Validation of Cilnidipine and Metoprolol Succinate in Combined Dosage Form

摘要

Introduction and Objectives: The present work involves developmentand validation of HPTLC method for simultaneous estimation of Cilnidipineand Metoprolol Succinate in their combined tablet dosage form. Method:In HPTLC method, Silica Gel G60 F254 TLC plate as the stationary phaseand a mobile phase of Toluene: Chloroform: Methanol: Glacial acetic acid(45: 25: 25: 5 v/v/v/v) was used to resolve CIL and METO. CIL and METOwere quantified at 231 nm. The proposed method weas validated accordingto International Conference on Harmonization. Result and Discussion:Two well-separated and sharp peak for CIL and METO were obtained at Rfvalues of 0.70 ± 0.01 and 0.34 ± 0.005 respectively. The linearity range obtainedfor HPTLC method were 100-500 ng/spot and 500-2500 ng/spot forCIL and METO respectively. Conclusion: Method validation was found tobe accurate, specific and precise.The developed method was successfullyapplied for estimation of CIL and METO in combined tablet formulation.