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Swine trichinellosis in slaughterhouses of the metropolitan area of Toluca

机译:托卢卡市区屠宰场的猪旋毛虫病

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In order to determine the prevalence of Trichinella spiralis infections in abattoirs of the metropolitan area of Toluca where pigs from commercial farms as well as backyard pigs are slaughtered, 539 swine diaphragm tissue samples were collected and examined by trichinoscopy and artificial digestion. Serum samples from the same animals were analyzed by ELISA using somatic and excretory/secretory antigens, and by Western blot analysis. T. spiralis muscle larvae were not found by trichinoscopy or artificial digestion. However, specific antibodies were detected by ELISA and confirmed by Western blotting in 12.4 % of the serum samples examined. Analysis of risk factors showed no association of seropositive results with sex. However, significant higher risk was observed in swine seven to 12 months old and in backyard pigs, compared with pigs from commercial farms.Key words: trichinellosis / swine / seroprevalence / risk factors
机译:为了确定在屠杀来自商业农场的猪和后院猪的托卢卡市屠宰场中旋毛虫的感染率,收集了539个猪diaphragm膜组织样本,并通过旋毛虫镜和人工消化进行了检查。使用体细胞和排泄/分泌抗原通过ELISA分析来自同一动物的血清样品,并通过Western印迹分析。旋毛虫镜或人工消化未发现螺旋螺旋肌幼虫。但是,通过ELISA检测到特异性抗体,并通过蛋白质印迹法在所检查的12.4%的血清样品中确认。危险因素分析表明,血清阳性结果与性别无关。然而,与商业农场的猪相比,在7至12个月大的猪和后院猪中观察到明显更高的风险。关键词:旋毛虫病/猪/血清阳性率/危险因素

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