Metabolism and Aging: From Molecular Physiology to Systems Biology

摘要

We utilized trans-mitochondrial hybrids (cybrids) of LHON carrying ND4/ND6/ND1 mutations grown in regular and mitochondrial challenged conditions. We investigated polar metabolites profiling in the cell extracts using LC-MS approach and found alterations in various metabolic intermediates of pentose phosphate pathway, amino acid metabolism, fatty acid metabolism, purine metabolism, glycolysis, and TCA cycle.