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In silico identification and characterization of cumulative abiotic stress responding genes in potato (Solanum tuberosum L.)

机译:马铃薯(Solanum tuberosum L.)中累积的非生物胁迫响应基因的计算机鉴定和表征

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Potato (Solanum tuberosum) is the world's fourth largest food crop, following rice, wheat, and maize. In potato abiotic stresses commonly reduce both yield and quality. The understanding of plant responses to abiotic stresses at transcriptome level provides a foundation for the identification of stress responding genes. The cumulative abiotic stresses genes, responding to three or more than three abiotic stresses, will be a good source of candidate genes for engineering and developing the abiotic stress resistant food crops. In potato various studies have been made for the analysis of gene expression under different abiotic stress conditions, using DNA microarray technology. These available microarray data under abiotic stresses like; salt, cold, heat and drought were analyzed by applying the bioinformatic tools. Total 217 cumulative abiotic stresses responding genes were identified and characterized at different stressed stages after the study of 64896 ESTs. The abiotic stresses responsive genes were characterized on the basis of their function and validated using Arabidopsis thaliana as reference species.
机译:马铃薯(Solanum tuberosum)是世界第四大粮食作物,仅次于水稻,小麦和玉米。在马铃薯中,非生物胁迫通常会降低产量和质量。在转录组水平上对植物对非生物胁迫的响应的理解为鉴定胁迫响应基因提供了基础。累积的非生物胁迫基因响应三种或三种以上的非生物胁迫,将成为候选基因的良好来源,可用于工程和开发抗非生物胁迫的粮食作物。在马铃薯中,已经进行了各种研究,以利用DNA微阵列技术分析不同非生物胁迫条件下的基因表达。这些在非生物胁迫下可获得的微阵列数据如;通过应用生物信息学工具分析了盐,冷,热和干旱。在对64896个EST的研究中,共鉴定了217个累积的非生物胁迫响应基因,并在不同胁迫阶段进行了表征。非生物胁迫响应基因根据其功能进行表征,并使用拟南芥作为参考物种进行验证。

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