Glucose detection using BSA:PEDOT-PSS as bioactive solute and solid bioactive layer deposited by spin coating

摘要

The two glucose-protein-polymer composite solutions, Glucose: BSA:PEDOT-PSS [10:1:1] and Glucose/BSA:PEDOT-PSS [1:1], were prepared by mixing protein (BSA), polymer (PEDOT-PSS) and glucose solutions for the first sample and protein-polymer-composite (BSA:PEDOT-PSS [1:1]) and glucose solutions for the second sample with both pH and concentration in biological range values. An analysis of the UV-Vis spectrum of the Glucose/BSA:PEDOT-PSS [1:1] solution to determine the glucose concentration and a comparative study using UV-Vis and ATR/FTIR absorption spectra recorded at room temperature for BSA, PEDOT-PSS, BSA:PEDOT-PSS [1:1] and Glucose:BSA:PEDOT-PSS[10:1:1] were performed. Afterwards, both the Glucose: BSA:PEDOT-PSS [10:1:1] and Glucose/BSA:PEDOT-PSS [1:1] solutions were deposited as solid bioactive layer on silicon (100) coated with a silver thin layer by PLD technique. AFM (topography profiles, electrostatic images, F - z curves) and ATR/FTIR techniques were used to identify morphological and compositional characteristics of the solid bioactive layers surface.