Snake venom neutralising factor from the root extract of Emblica officinalis Linn.

摘要

The folk use of the Indian medicinal plant Emblica officinalis Linn. root extract and its active compound (Pthalate in nature) against snake venom has been established in experimental animal models. From the root extract of Emblica officinalis, a compound was isolated through silica gel column chromatography and the structure was determined by UV, IR, 1H-NMR, 13C-NMR & EIMS studies. The structure was determined and the active fraction was a pthalate compound, which could neutralize viper and cobra venom induced lethal, hemorrhagic, defibrinogenating, proinflammatory, PLA2, cardiotoxic and neurotoxic activity in experimental animal models. Viper venom induced myotoxicity was antagonized by the compound as shown by the decreased levels of the myotoxicity marker enzymes CPK & LDH. The pro-inflammatory action of viper venom neutralized by the active compound, showed the involvement of the pro-inflammatory markers IL-1, IL-6, TNF-α. The present study confirmed the phytomedicinal value of an antisnake venom compound present in the root of Emblica officinalis.