Fluorometric Method for the Determination of Gemifloxacin Mesylate in Bulk and Pharmaceutical Formulations Using Tbsup3+/sup Ions in the Presence of Hexamine

摘要

A simple and highly sensitive method for the determination of Gemifloxacin Mesylate in bulk drug and pharmaceutical formulations. The method is based on the reaction of Gemifloxacin Mesylate with Tbsup3+/sup in the presence of hexamine at pH 7.2. The fluorescence was measured at 545 nm after excitation at 271 nm. The calibration graph was linear over the range 0.05–1.3 µg mL, with minimum quantification limit (LOQ) of 0.024 µg mL and minimum limit of detection (LOD) of 7.65×10sup-3/sup µg mL. The proposed method was successfully applied for the determination of this drug in its dosage forms.