d-@a-tocopherol nanoemulsions: Size properties, rheological behavior, surface tension, osmolarity and cytotoxicity

摘要

The aim of this study was the assessment of the physicochemical stability of d-@a-tocopherol formulated in medium chain triglyceride nanoemulsions, stabilized with Tween(R)80 and Lipoid(R)S75 as surfactant and co-surfactant, respectively. d-@a-tocopherol was selected as active ingredient because of its well-recognized interesting anti-oxidant properties (such as radical scavenger) for food and pharmaceutical industries. A series of nanoemulsions of mean droplet size below 90nm (polydispersity index0.15) have been produced by high-pressure homogenization, and their surface electrical charge (zeta potential), pH, surface tension, osmolarity, and rheological behavior, were characterized as a function of the d-@a-tocopherol loading. In vitro studies in Caco-2 cell lines confirmed the safety profile of the developed nanoemulsions with percentage of cell viability above 90% for all formulations.