Anticandidal activity of the extract and compounds isolated from Cyperus conglomertus Rottb

摘要

The phytochemical screening of Cyperus conglomeratus showed that carbohydrates and/or glycosides, flavonoids, tannins, sterols and/or triterpenes, and proteins and/or amino acids are present. The fatty acid profile comprised major; palmitic, oleic, heptadecanoic, linoleic and minor; arachidonic, lignoceric, stearic, and myristic acid. Two compounds; namely, α-amyrin and β-sitosterol were isolated by the fractionation of unsaponifiable matter.The acute toxicity study showed that the reported after oral administration of the alcohol extract ( TAE) showed that the plant was highly safe as the LD50 was more than 4000?mg/kg. These results were well supported by the sub-chronic toxicity, as the TAE administrated to rats for 15 consecutive days at dose 1000?mg/kg showed no alteration in the liver and kidney functions. Moreover, the extract of the plant exhibited anti-candidal activity against different Candida species. The most potent activity, (23.1?±?2.1, 0.98?μg/ml) and (22.3?±?0.53, 0.98?μg/ml), was obtained by the chloroform and total extract, respectively against Candida albicans .