HS-GC Method Validation for Determination of Residual Solvents In Herbal Extracts

摘要

Residual solvent in herbal extracts were monitored using head space gas chromatograph (HS-GC) with fl ame ionization detector (FID) and headspace AOC 5000 auto sampler. The separation of the residual solvents was achieved on a Zebron (ZB-624) column (30 m X 0.25 mm i.d. X 0.25 μm coating thickness) using GC 2010 Shimadzu, with nitrogen as a carrier gas in the split mode by headspace method. Fast separation of 7 commonly used solvents (methanol, ethanol, acetone, n-hexane, ethyl acetate, ethylene dichloride and toluene) was achieved in a single analysis of 9.19 min run time with 1.95 ml/min fl ow rate. The oven and detector temperature was 34°C and 255°C respectively using N,N’-Dimethylformamide(DMF) as a diluent. The method was successfully validated for linearity, specifi city, precision, accuracy, and range with good recoveries. The linear range for standard mix solvent was 10 to 200 ppm.