As a sustainable mode of transportation, bicycles significantly improve daily mobility. In order to provide theoretics support for improvement of the bicycling environment, this paper proposed bicycle level of service (BLOS) evaluation method for urban road segment according to cyclists’ perception. First, influence factors of BLOS were identified from aspects of road facility, traffic characteristics, and environmental condition. Second, bicycling videos were recorded and a satisfaction survey was conducted. Four BLOS evaluation models for different separation facilities were established. Last, bicycling behavioral stages of travelers were divided based on the transtheoretical model. A new BLOS classification criterion was proposed according to travelers’ demand of different stages.
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