Sequence Characterization of Coding Regions of the Myostatin Gene (GDF8) from Bakerwal Goats (Capra hircus) and Comparison with the Sheep (Ovis aries) Sequence

摘要

The Bakerwal breed of goat in Kashmir valley is a good meat purpose breed of goat. That attains an appreciable body weight under a low input production system. As these goats are mainly reared by Gujjar and Bakarwal tribes of the J & K state, so they usually are fed with the weeds, herbs, shrubs and grasses of pastures that will otherwise go waste. These goats constitute the main livestock wealth. With the good productive and reproductive potential, it makes these animals an important animal protein source for developing countries like India. The myostatin gene (GDF8) is important in the physiology of stock animals because its product produces a direct effect on muscle development and consequently also on meat production. The myostatin sequence is known in several mammalian species and shows a high degree of amino acid sequence conservation, although several alterations in the intron and exon regions have been identified. The objective of our work is to characterize the myostatin coding regions using gene sequencing and polymerase chain reaction methods of Capra hircus (Bakerwal breed) and to compare them with the Ovis aries and other livestock species of animal, looking for variations in nucleotide and protein sequences. As mutations in the myostatin gene can inactivate its expression and result in a non-functional protein, which leads to increase in muscle growth in many species. In this way, we are able to identify 3 alterations on the presumed myostatin protein sequence as compared to non double-muscled ovine sequences.