Concentration of the bioactive principles in Geranium robertianum extracts through membranare procedures (ultrafiltration)

摘要

and 15% (mass concentration) hydro-alcoholic extracts of Geranium robertianum in 50%, 70% and 96% alcohol were obtained. They were further purified and concentrated through membranare procedures (ultrafiltration). 2 types of membranes were used: Millipore ultrafiltration membranes of regenerated cellulose with 10.000 Da cut-off and composite membrane: polysulphona— polyaniline - PSF/PANI. The contents of flavones, total polyphenols, proteins and reducing sugars in all extracts’ types ranged between: 7.233 — 72.71 mg/mL -flavones, 271.17 — 437.07 ug/mL — polyphenols, 1.262 — 2.40 ug/mL -proteins and 153.10 — 661.60 ug/mL —sugars. It was ascertained that such contents were proportional to plant mass and to the used solvent concentration, the maximum being obtained in extracts realized in 70% ethanol, with 10% plant mass. The increase of ethanol concentrations and plant mass above the mentioned values resulted in the decrease of the concentration of the studied compounds.