The use of RAPD and ISSR markers for genetic diversity among some barley cultivars

摘要

The success of a breeding program depended on the genetic variability available into the germoplasm of the crop. The objectiveof the present study were to assess molecular variation among different Romanian and foreign barley cultivars, and to determine the level of genetic similarity among them. The results demonstrated that RAPD analysis are useful for evaluation of genetic diversity between different barley cultivars, considering the fact that the average polymorphic rate was 91,17 %, and 7,5 polimorphyc bands /primer. Total polymorphism generated by a certain primer (PIC), presented values between 0,147 to P27 and 0,438 to P-1. The discrimination index (PI), registered values among 0,787 for the primer E6 and 2,626 for P-1 primer, which had the highest capacity to generate polymorphic bands to barley cultivars studied. Among the tested ISSR primers, only 5 amplified polymorphic ISSR loci with an average number of 9.2 bands/primer and the mean percentage of ISSR polymorphism was 89,13%. The total of polymorphism (PIC) presented values contained among 0,245 to 811 and 0,391 to 810. The (PI) registered values among 1,562 for primer 810 and 2,803 for HB15 primer. RAPD-PCR and ISSR analysis through two categories of markers, attested the existence of a high genetic variability among studied cultivars, which can be efficiently exploited in the breeding programs of barley. The use _ of cultivars from various clusters and sub clusters offer the possibility of obtaining an appropriate genetic variability in hybrid population.