Biodegradation of karathane using adapted Pseudomonas aeruginosa in scale up process

摘要

Nowadays, one of the major problems facing the industrialized world is the contamination of soils, groundwater and sediments with pesticides. Despite the valuable contributions associated with the use of pesticides, many of these biologically active chemicals represent a potential hazard to humans and to nature. In this study, biodegradation potential of adapted Pseudomonas aeruginosa S3 has been assessed on karathane (active ingredient dinocap), a contact fungicide and a non-systemic acaricide. The strain was isolated from contaminated soil and adapted to grow on karathane (up to 1%) as the only carbon source that is converted into less toxic and environment friendly compound. The media and process optimization was carried out in order to scale up the process. The higher biomass production was obtained on mineral salt media with corn flour 1% (nitrogen source) and glucose as additional carbon source. Also, the effect of initial pH and inoculum volume on biomass growth has been studied. The biodegradation potential of Pseudomonas aeruginosa S3 has been tested on soil contaminated with 0.2% karathane where the strain degradated 45.8% of pesticide.