Wound Healing Properties of Ziziphus jujuba Mill. leaves

摘要

A dry ethanolic extract was obtained from Ziziphus jujuba Mill. leaves. To select the best solvent and extraction method for flavones and polyphenol-carboxylic acids, initially was applied a multiple linear regression model with the method and solvents as dummy variables, which indicated the largest positive influence on extraction for ethanol 70% and the largest negative effect for sonication at room temperature. Then, was dropped out the sonication method and was applied a simple linear regression model with the solvent as an independent variable for reflux extraction. The extract embedded in simple ointment (10% w/w) was evaluated in rat for wound healing activity by topical application for a period of 12 days. The animal experiment was carried out using Cicat (a known commercial ointment) as a positive control. The animals treated with Z. jujuba leaf extract showed a healing of 82.25% compared to the beginning of the experiment. The healing effect was similar to Cicatrizin (83.10%) and slightly higher, but not statistically significant from the one seen in the negative control group (76.2%, p=0.259) at the end of the study. Complete healing occurred afier 18 days in both groups, without obvious scars and after 20 days in the control group.