Abrasion stimulations on biological behaviors of fascial fibroblasts

摘要

Objective: To investigate the influence of different in vitro abrasions on cell viability and protein expression and to provide experimental foundation for cellular and biophysical mechanism of basic mechanical stimulation via acupuncture and tuina. Methods: Fibroblasts from the meridian-related fascial connective tissue were cultured in vitro, and abrasion was applied onto the study subjects with different intensities. The deviations of cell viability and protein expression in the fibroblasts were observed. Results: We found that abrasions may decrease the viability of fibroblasts from the meridianrelated fascia, resulting in reduced cell viability rate and roundness. Abrasion intensity is the factor that inhibits the cell viability rate and roundness, while the number of stimulations is not. These two factors (abrasion intensity and the number of stimulations) are not integrative. Furthermore, the abrasion stimulations from all 3 groups result in a clear adjustment of protein expression of the fibroblasts. We found that abrasion stimulations with medium intensity have maximum effect on the protein expression, as supported by the clustering analysis. Conclusions: We found that abrasion stimulations with medium intensity may have maximum role in inhibition of cell viability and protein synthesis/adjustment.