Influence of culture medium components on Mycoplasma gallisepticum growth

摘要

The composition of a culture medium produced in Cuba to grow Mycoplasma gallisepticum was evaluated using a response surface methodology. Initially, the influence of Mycoplasma base, horse serum and yeast extract on biomass production was assessed in a 23 factorial experimental design. Then, a central composite design was applied. Mycoplasma base and horse serum factors were selected for this study. In 7 L and 35 L bioreactors, the variations carried out in the culture medium composition were estimated. The experimental results were fitted to a second order polynomial model equation. Finally, the optimal growth conditions established were at concentrations of 1,88% Mycoplasma base and 18,16% horse serum (v/v) with 0,6% of yeast extract. For the conditions tested, at a bioreactors scale, a high cellular yield of M. gallisepticum (higher than 0,5 ODu/mL) was obtained.