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Efeitos da biocimenta??o promovida por Bacillus subtilis nas propriedades mecanicas de argamassas

机译:枯草芽孢杆菌促进生物胶凝作用对砂浆力学性能的影响

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This study aims to evaluate the influence of B. subtilis AP91 spores addition on the mechanical properties of mortars. B. subtilis strain AP91, isolated from rice leaves of the needle variety, which has an early cycle of production, was used at the concentration of 105 spores/mL in mortars with cement-to-sand ratio of 1:3 (by weight) and water-to-cement ratio (w/c) of 0.63. These spores were added in two different ways: in the mixing water and by immersion in a solution containing bacterial spores. Scanning Electron Microscope (SEM) analysis showed crystals with calcium peaks on the EDS, which possibly indicates the presence of bioprecipitated calcium carbonate. The results obtained in the mechanical analysis showed that the bioprecipitation of CaCO 3 by B. subtilis strain AP91 was satisfactory, particularly when the spores were added in the mixing water, increasing the compressive strength up to 31%. Thus, it was concluded that the addition of B. subtilis AP91 spores in the mixing water of cement mortars induced biocementation, which increased the compressive strength. This bioprecipitation of calcium carbonate may very well have other advantageous consequences, such as the closure of pores and cracks in cementitious materials that could improve durability properties, although more research is still needed on this matter.
机译:这项研究旨在评估枯草芽孢杆菌AP91孢子添加对砂浆力学性能的影响。从针叶品种的稻叶中分离的枯草芽孢杆菌AP91菌株具有较早的生产周期,在砂浆中以105孢子/ mL的浓度使用,水泥与砂的比例为1:3(按重量计)水灰比(w / c)为0.63。这些孢子以两种不同的方式添加:在混合水中和浸入含有细菌孢子的溶液中。扫描电子显微镜(SEM)分析显示在EDS上具有钙峰的晶体,这可能表明存在生物沉淀的碳酸钙。在机械分析中获得的结果表明,枯草芽孢杆菌AP91对CaCO 3的生物沉淀是令人满意的,特别是当在混合水中添加孢子时,其抗压强度提高了31%。因此,可以得出结论,在水泥砂浆的混合水中添加枯草芽孢杆菌AP91孢子会引起生物胶凝作用,从而提高了抗压强度。碳酸钙的这种生物沉淀可能会带来其他有利的后果,例如封闭胶结材料中的孔和裂缝,这可以改善耐久性,尽管对此问题仍需要进行更多的研究。

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