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首页> 外文期刊>Revista de microbiologia >Molecular mechanism of fluoroquinolones resistance in Mycoplasma hominis clinical isolates
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Molecular mechanism of fluoroquinolones resistance in Mycoplasma hominis clinical isolates

机译:人型支原体临床分离株对氟喹诺酮类药物耐药的分子机制

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To evaluate the molecular mechanism of fluoroquinolones resistance in Mycoplasma hominis (MH) clinical strains isolated from urogenital specimens. 15 MH clinical isolates with different phenotypes of resistance to fluoroquinolones antibiotics were screened for mutations in the quinolone resistance-determining regions (QRDRs) of DNA gyrase (gyrA and gyrB) and topoisomerase IV (parC and parE) in comparison with the reference strain PG21, which is susceptible to fluoroquinolones antibiotics. 15 MH isolates with three kinds of quinolone resistance phenotypes were obtained. Thirteen out of these quinolone-resistant isolates were found to carry nucleotide substitutions in either gyrA or parC. There were no alterations in gyrB and no mutations were found in the isolates with a phenotype of resistance to Ofloxacin (OFX), intermediate resistant to Levofloxacin (LVX) and Sparfloxacin (SFX), and those susceptible to all three tested antibiotics. The molecular mechanism of fluoroquinolone resistance in clinical isolates of MH was reported in this study. The single amino acid mutation in ParC of MH may relate to the resistance to OFX and LVX and the high-level resistance to fluoroquinolones for MH is likely associated with mutations in both DNA gyrase and the ParC subunit of topoisomerase IV.
机译:为了评估从泌尿生殖道标本分离的人支原体(MH)临床菌株中氟喹诺酮类药物耐药的分子机制。与参考菌株PG21相比,筛选了15株对氟喹诺酮类抗生素具有不同表型耐药性的临床分离株,以检测DNA促旋酶(gyrA和gyrB)和拓扑异构酶IV(parC和parE)的喹诺酮耐药性决定区(QRDRs)中的突变,对氟喹诺酮类抗生素敏感。获得了具有三种喹诺酮抗性表型的15株MH分离株。发现这些喹诺酮耐药菌株中有13个在gyrA或parC中带有核苷酸取代。在具有对氧氟沙星(OFX)耐药,对左氧氟沙星(LVX)和司帕沙星(SFX)耐药的表型以及对所有三种抗生素都敏感的菌株中,gyrB没有变化,也没有发现突变。本研究报道了MH临床分离株中氟喹诺酮耐药的分子机制。 MH的ParC中的单个氨基酸突变可能与对OFX和LVX的抗性有关,MH对氟喹诺酮类药物的高水平抗性可能与DNA促旋酶和拓扑异构酶IV的ParC亚基的突变有关。

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