The influence of a magnetic field on photon beam radiotherapy in a normal human TK6 lymphoblastoid cell line

摘要

The implementation of magnetic resonance imaging (MRI) guided radiotherapy (RT) continues to increase. Very limited in-vitro data on the interaction of ionizing radiation and magnetic fields (MF) have been published. In these experiments we focused on the radiation response in a MF of the TK6 human lymphoblastoid cells which are known to be highly radiosensitive due to efficient radiation-induced apoptosis. Clonogenicity was determined 12–14?days after irradiation with 1–4?Gy 6 MV photons with or without a 1.0 Tesla MF. Furthermore, alterations in cell cycle distribution and rates of radiation induced apoptosis (FACS analysis of cells with sub-G1 DNA content) were analyzed. Clonogenic survival showed an exponential dose-dependence, and the radiation sensitivity parameter (α?=?1.57/Gy) was in accordance with earlier reports. Upon comparing the clonogenic survival between the two groups, identical results within error bars were obtained. The survival fractions at 2?Gy were 9% (without MF) and 8.5% (with MF), respectively. A 1.0 Tesla MF does not affect the clonogenicity of TK6 cells irradiated with 1–4?Gy 6MV photons. This supports the use of MRI guided RT, however ongoing research on the interaction of MF and radiotherapy is warranted.