Detection of Cyclospora, Microsporidia and Cryptosporidium by direct microscopy and PCR in stools specimens in Northwest, Iran

摘要

This study was designed to detect the presence of Cyclospora, Microsporidia and Cryptosporidium by several different methods. A total of 1825 fecal samples submitted to the Central Parasitology Laboratory of Tabriz were assessed by conventional methods of light microscopy, formalin-ether concentration technique, staining technique and PCR method. The frequency of Cryptosporidium was 0. 0%, 15%, 3% and 2% and Microsporidia was 0. 0%, 3%, 1% and 0% by methods of microscopy, formalin-ether concentration, staining and PCR, respectively. Cyclospora was not found in all the stool samples examined. The frequencies of these three protozoa in our study were less than our expectations. Lack of infection with Cyclospora can be good news for local health care providers. More investigations especially in at risk populations is needed for health policy making.