Evaluation of anti-helminthic activity of ethanolic extract of withania coagulans dunal (flower buds)

摘要

Withania coagulans is a short shrub with central stem. It has milk coagulating property and various parts of this plant were traditionally used as Emetic, Diuretic and Anti diabetic agent. It is also used in chronic liver failure. Berries of this plant contain amino acids, fatty and essential oil and alkaloids and also show anthelmintic activity. Hence the flower Buds of this plant were examined for anti-helminthic activity using earthworms (Pheretima posthuma). Numerous concentrations (25mgml-1, 50mgml-1, 75mgml-1 and 100mgml-1) of ethanolic extract of Withania coagulans (EEWC) were studied. Albendazole (20mgml-1) was used as standard drug for reference whereas normal saline (NS) as control. The time of paralysis and death of worms were determined and recorded. Extract exhibit significant anti-helminthic activity at the concentration of 75 and 100mgml-1. The result shows that ethanolic extract of Withania coagulans possesses vermicidal activity against earthworms (Pheretima posthuma) and determined to be effective as anti-helminthics. Therefore, the anti-helminthic activity of the ethanolic extract of Withania coagulans dunal (Flower Buds) has been reported.