Isolation of Salmonella enteritidis Using Biochemical Tests and Diagnostic Potential of SdfI Amplified Gene

摘要

Salmonella enteritidis is a food borne pathogen that affects the human health by infecting poultries. The aim of this study is to set up methods for identifying S. enteritidis, based on molecular properties of this bacterium. In this study, two control isolates of RTCC1623 and RTCC1624, obtained from the institute of Razi (Karaj, Iran) and 76 Kermanshah poultry derived samples were isolated using biochemical tests. Genomic DNA was extracted by universal Phenol-Chloroform method and PCR was performed via the specific primers of SdfI-F and SdfI-R of the SdfI (AF370707) gene. Amplified fragments of the 333 (SdfI) base pairs were observed in 66 of the total 76 S. enteritidis isolates. This study recommends that the identification of these pathogens by PCR method can be replaced with traditional bacteriological techniques. The PCR method is a rapid approach for recognizing and identifying the S. enteritidis infections in chicken products.