An efficient regeneration system via somatic embryogenesis in some egyptian durum wheat cultivars mediated high-throughput transformation of durum wheat using Agrobacterium tumefaciens

摘要

In this study, an efficient Agrobacterium-mediated transformation protocol for elite Egyptian durum wheatcultivars was established. The obtained results revealed that, the influence of dicamba on induction ofembryogenic calli with somatic embryos was significantly high in comparison with 2,4-D and picloram in alltested cultivars. Thus, the callus induction medium (CIM1) supplemented with 2mg/l dicamba considered to bethe optimum induction medium for somatic embryogenesis and high regeneration capacity in differentgenotypes of Egyptian durum wheat (Banisweef 1, Banisweef 4, Banisweef 5, Banisweef 6, Sohag 2 and Sohag3). Thus, these results proved that, this protocol is less genotype-dependent plant regeneration system and thishigh level of regeneration in particular with cv. Banisweef 6 (89.66 %) and cv. Sohag 3 (83.66 %) was enabledto attain high frequencies of transformation efficiency ranged from 4.93 % to 14.20 % with an average value10.06 % according to (Total No. of positive plants/No. of explants) deduced from 10 independent experimentsusing 1503 explants (scutellum of immature embryos) of cv. Banisweef 6. In Comparison to the deduced resultsfrom 10 independent experiments using 1583 explants of cv. Sohag 3 with transfornation frequencies rangedfrom 2.58 % to 10.18% with an average value 6.73%. The obtained results in this study will sustain furtherdevelopment of transgenic plants of other commercial durum and bread wheat cultivars and other cereal cropssuch as maize, rice and sorghum.