Methanolic extraction and isolation of bioactive chemicals from Pithecellobium dulce leaves by column chromatography and GC-MS studies

摘要

Pithecellobium dulce is a species family Fabaceae, that is native to the India mostly Maharashtra area of Vidarbha. Pithecellobium dulce found secondary metabolism. It is used as medicinal plant. The methanolic extract of leaves was obtained by Soxhelt extractor followed by concentration in rotary evaporator. Separation of bioactive chemicals was carried out by column chromatography while studies by GC-MS which shows presence of following bioactive chemicals Squalene,9-Octadecenoic acid(Z)-,2-hydroxy-13propanediyl ester; 9 9-Octdecenoic acid,1,2,3-propanetriyl ester.