Polyethyleneimine-modified calcium carbonate nanoparticles for p53 gene delivery

摘要

In this study, calcium carbonate (CaCO₃) nanoparticles with spherical structure were regulated by arginine and successfully synthesized via a facile co-precipitation method. The average particle size of as-prepared CaCO₃ was about 900?nm. The properties of nanostructured CaCO₃ particles were characterized by scanning electron microscope, Fourier transform infrared spectroscopy, X-ray diffraction and size distribution. After modified with polyethyleneimine (PEI), the ability of PEI-CaCO₃ nanoparticles to carry GFP-marked p53 gene (pEGFP-C1-p53) into cancer cells to express P53 protein were studied. Meanwhile, the cytotoxicity, transfection efficiency, cells growth inhibition and the ability to induce apoptosis by expressed P53 protein were conducted to evaluate the performances of PEI-CaCO₃ nanoparticles. The results show that prepared PEI-CaCO₃ nanoparticles had good biocompatibility and low cytotoxicity in a certain concentration range. PEI-CaCO₃ effectively transfected pEGFP-C1 gene into epithelial-like cancer cells. And with the expression of GFP-P53 fusion protein, pEGFP-C1-p53-gene-loaded PEI-CaCO₃ particles significantly reduced the proliferation of cancer cells. These findings indicate that our PEI-modified CaCO₃ nanoparticles are potential to be successfully used as carriers for gene therapy.