聚乙烯亚胺修饰的银纳米颗粒的制备研究及在肝素检测中的应用

摘要

We have synthesized fluorescent silver nanoparticles by using polyethyleneimine and sodium borohy-dride under ambient conditions by using chemical reduction method. This method was easy, convenient and repeat-able. The properties of these novel sliver nanoparticles were characterized using included UV-visible spectrometry, dynamic light scattering and transmission electron microscopy. By using these silver nanoparticles, we established a highly sensitive and selective colormetric sensing method for heparin detection, and the low limit of detection was found to be 1μg/mL.%该文利用分子量为600的高支化聚胺高分子PEI，在室温条件下，采用化学还原法，使用还原剂硼氢化钠，合成了银纳米颗粒。该方法具有操作简单、便捷、可重复性高等特点。对合成的颗粒进行了一系列的表征，包括紫外可见光谱的测量，动态光散射测量和透射电镜表征等。并以该银纳米颗粒为基础，建立了比色法传感器用于肝素的快速检测，并获得了较好的检测结果，检测限为1滋g/mL。该检测方法具选择性高、灵敏度好，且检测耗时短，便于人眼直接观测等优点。