The performance of field collected flies (Glossina pallidipes and Glossina brevipalpis) from Msubugwe game reserve for colony build up at Vector and Vector Borne Disease laboratories in Tanzania

摘要

Area Wide Integrated Pest Management (AW-IPM) programme using Sterile Insect Technique (SIT) depends on a reliable supply of large numbers of high quality sterile insect for release. Vector and Vector Borne Disease Institute (VVBD) is currently rearing G. pallidipes, G. austeni and G. brevipalpis mainly for research and eradication purposes. However, the production of the species mentioned above is still low, therefore the institute decided to start collection of wild flies for the purpose of boosting their production in the laboratory. The collection of wild flies was done at Msubugwe game reserve in Pangani district. A total of 31 pyramidal traps baited with acetone and cow urine were deployed in the collection area. The average catches per day were 200 female G. pallidipes and 10 female G. brevipalpis. Immediate after collection flies were fed on goats then transported to the institute for rearing. A total of 6,038 female of G. pallidipes and 911 female of G. brevipalpis were adapted in the laboratory. In the laboratory, G. brevipalpis had an average mortality of 1.3± 0.23% with fecundity of 0.6± 0.05 per week and G. pallidipes had an average mortality of 3.1 ± 0.32% with fecundity of 0.2± 0.03 per week. The mortality of G. brevipalpis was slightly higher (P 0.001) than the recommended which is ≤ 1% but its fecundity was very good as it was above the recommended fecundity. However G. pallidipes had significant higher (P 0.001) mortality with very low fecundity. The average pupae produced per week by G. brevipalpis was 48± 9.71 from an average of 485 females and that of G. pallidipes was 25± 2.77 from an average of 57 females. Generally G. brevipalpis performed better than G. pallidipes in the laboratory; there was significant increase on the production of the specie in the laboratory.The poor performance observed in G. pallidipes could be due to viral ( Salivary gland hyperplasia virus) infection which is known to affect the reproductive performance of the specie.