Antileukemic Effect of Polyphenol of Local Grape Seeds Extract from Duhok/Kurdistan of Iraq: in vitro Study

摘要

The present study was conducted to evaluate the cytotoxic effect of secondary metabolites extract (polyphenol) from local grape seeds of vitis viniferra (rash meu) from Duhok Governorate / Kurdistan of Iraq using MTT assay. Different concentrations 39.1 μg/ml, 78.125 μg/ml, 156.25 μg/ml, 312.5 μg/ml μg/ml 625 μg/ml, μg/ml 1250 μg/ml, and 2500μg/ml of polyphenol extract were tested against the proliferation of acute leukemic lymphocytes of Acute Myeloid Leukemia (AML) and acute Lymphoid Leukemia (ALL) patients in culture within 24 and 48 hours exposure. This study showed that a polyphenolic extract that isolated from seeds of local grape is substantially rich in phenolic content and flavonoid. The result of cytotoxicity assay revealed that all concentrations of polyphenol extract were effective against the proliferation of both types of acute leukemic lymphocytes of AML and ALL patients. Statistical analysis for all patients with AML and all patients with ALL was revealed that the higher concentrations 1250μg/ml and 2500μg/ml were effected after both time of exposure 24 and 48hours. The result of cytotoxicity assay showed that the highest concentration 2500μg/ml was more effected after 24 and 48 hours of all acute leukemic cases. The CC50 value was 859μg/ml after 24hours of treatment. The result of present study showed no significant different between two type of acute leukemia AML and ALL in to response to cytotoxic effect of polyphenol extract.