The effects of dietary probiotics on natural IgM antibody titres of Kenyan indigenous chicken

摘要

ThisstudywasconductedtoinvestigatetheeffectsofprobioticsupplementationonserumnaturalIgMlevelsbindingkeyholelimpethemocyanininindigenouschicken(IC).Onehundredandfiftytwomonthsoldchickenraisedunderlowinput-outputsystemweresourcedfromsmallscaleindigenouschickenfarmersfromNyakachandEminengsubcountiesofKisumuandBaringo,Kenyarespectively.TheICwereofmixedsex,randomlydividedintofivetreatmentgroupsof25birdseach.Thetreatmentswere5mlofMolaplusdissolvedinto250,500,1000,1500and2000mlofdrinkingwater.Thebirdswereraisedintogroupcages,andfedcommercialgrowermashfortwomonthsduringthestudyperiod.Awindowof14dayswasleftforimmunestabilization,bloodwasthendrawnfromthewebveinandserumseparatedimmediately.LevelsofIgMbindingwasassayedusinganindirectELISAtechnique.IgMbindingKLHwasfoundbutdietaryprobioticsupplementationsdidnotsignificantlyaffectlevelsofIgMbindingKLHintheserum.ProbioticsupplementationinthedietdidnotfurtherenhanceKLHbindingIgMinICrearedundervillageproductionsystem.