Effect of Topical Resveratrol Formulation on Healing of Experimental Full Thickness Wound in Diabetic Male Albino Rats: (Histological and Immunohistochemical study)

摘要

Impaired healing of wounds is one of the most serious diabetes mellitus microvascular complications. Resveratrol (RSV), a natural polyphenol compound, has antioxidant, anti-inflammatory and antidiabetic activities. Our study aimed to evaluate the potential of topical resveratrol formulation on histological, immunohistochemical and anti-oxidative findings in experimentally induced full-thickness wound model in induced diabetic rats. Seventy - two albino rats were divided into two main groups: normal control group (18 rats) and diabetic group (54 rats) which divided equally into three sub groups: sub group (I) received plain ointment base and considered as control diabetic positive; sub group (II) received topical standard marketed product for wound healing containing β-Sitosterol (MEBO) (0.25% w/w) and considered as reference group, while sub group (III) received topical Resveratrol 0.5% ointment, and considered as test group. Wound surface area (W.S.A) and oxidative enzymes, Histological and Immunohistochemical studies were carried out for each group throughout treatment period (1 st , 2 nd , and 3 rd weeks). The obtained results showed that topical resveratrol ointment showed more potential healing effect on diabetic wound than the reference product. Resveratrol has percent wound contraction rate high than the wound closure rate than MEBO. Wound treated with resveratrol (group II) showed re-epithelialization and increased epidermal thickness compared with rats in control diabetic. So, topical application of resveratrol ointment has potential effect on enhancing wound healing process in diabetic conditions, and this activity may be attributed – mainly – to its free- radical scavenging activity.