Effects of MC-LR on ROS level in human bronchial epithelia cells and Chinese hamster ovary cells

摘要

Microcystins (MCs) are a family of cyclic heptapetide endotoxins that are mainly produced by cyanobacterial blooms in various eutrophic inland waters worldwide. More than 90 different structural analogues of MCs have been identified, of which microcystin-LR (MC-LR) is the most common variant. The effects of MC-LR on reactive oxygen species (ROS) were measured by flow cytometry in Chinese hamster ovary (CHO) cells and human bronchial epithelial (HBE) cells. The results showed that 2.5, 5, 10μg/ml MC-LR significantly increased the production of ROS in not only CHO) cells but also (HBE) cells, suggesting that ROS production involved in the procession of reproductive toxicity and respiratory toxicity induced by MC-LR. So a series of intracellular oxidative stress reactions induced by ROS lead to apoptosis need be further researched.