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The sea urchin metallothionein system: Comparative evaluation of the SpMTA and SpMTB metal-binding preferences ☆

机译:海胆金属硫蛋白系统:SpMTA和SpMTB金属结合偏好的比较评估☆

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Metallothioneins (MTs) constitute a superfamily of ubiquitous metal-binding proteins of low molecular weight and high Cys content. They are involved in metal homeostasis and detoxification, amongst other proposed biological functions. Two MT isoforms (SpMTA and SpMTB) have been reported in the echinoderm Strongylocentrotus purpuratus (sea urchin), both containing 20 Cys residues and presenting extremely similar sequences, although showing distinct tissular and ontogenic expression patterns. Although exhaustive information is available for the Cd(II)-SpMTA complex, this including the full resolution of its 3D structure, no data has been reported concerning either SpMTA Zn(II) and Cu(I) binding properties, or the characterization of SpMTB at protein level. In this work, both the SpMTA and SpMTB isoforms, as well as their separate α and β domains, have been recombinantly synthesized in the presence of Zn(II), Cd(II) or Cu(II), and the corresponding metal complexes have been analyzed using electrospray mass spectrometry, and CD, ICP-AES and UV–vis spectroscopies. The results clearly show a better performance of isoform A when binding Zn(II) and Cd(II), and of isoform B when coordinating Cu(I). Thus, our results confirm the differential metal binding preference of SpMTA and SpMTB, which, together with the reported induction pattern of the respective genes, highlights how also in Echinodermata the MT polymorphism may be linked to the evolution of different physiological roles.Highlights? SpMTA shows a better performance when binding Zn(II) and Cd(II). ? SpMTB shows a better performance when binding Cu(I). ? The β domain is responsible for the lower divalent metal binding capacity of SpMTB. ? The α and β domains contribute to the enhanced capacity of SpMTB for Cu(I) binding.
机译:金属硫蛋白(MTs)构成了低分子量,高Cys含量的无处不在的金属结合蛋白的超家族。他们参与金属稳态和排毒,以及其他拟议的生物学功能。棘皮动物紫僵菌(海胆)中已经报道了两种MT同工型(SpMTA和SpMTB),尽管含有明显的组织和本体表达模式,但它们均含有20个Cys残基并呈现出极为相似的序列。尽管可以获得Cd(II)-SpMTA复合物的详尽信息,包括其3D结构的完整分辨率,但尚未报告有关SpMTA Zn(II)和Cu(I)结合特性或SpMTB表征的数据。在蛋白质水平上。在这项工作中,已经在Zn(II),Cd(II)或Cu(II)的存在下重组合成了SpMTA和SpMTB亚型以及它们各自的α和β结构域,并且相应的金属配合物具有使用电喷雾质谱,CD,ICP-AES和紫外可见光谱进行分析。结果清楚地表明,当结合Zn(II)和Cd(II)时,同工型A的性能更好;当与Cu(I)配位时,同工型B的性能更好。因此,我们的结果证实了SpMTA和SpMTB的金属结合偏好差异,再加上相应基因的报道诱导模式,凸显了在棘皮动物中MT多态性也可能与不同生理作用的演变有关。当结合Zn(II)和Cd(II)时,SpMTA显示出更好的性能。 ?当结合Cu(I)时,SpMTB显示出更好的性能。 ? β结构域负责SpMTB较低的二价金属结合能力。 ? α和β结构域有助于SpMTB增强与Cu(I)结合的能力。

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