Trypanosoma cruzi DNA replication includes the sequential recruitment of pre-replication and replication machineries close to nuclear periphery

摘要

In eukaryotes, many nuclear processes are spatially compartmentalized. Previously, we have shown that in Trypanosoma cruzi , an early-divergent eukaryote, DNA replication occurs at the nuclear periphery where chromosomes remain constrained during the S phase of the cell cycle. We followed Orc1/Cdc6, a pre-replication machinery component, and the proliferating cell nuclear antigen (PCNA), a component of replication machinery, during the cell cycle of this protozoon. We found that, at the G1 stage, Orc1/Cdc6 and PCNA are dispersed throughout the nuclear space. During the G1/S transition, Orc1/Cdc6 migrates to a region close to nuclear periphery. At the onset of S phase, PCNA is loaded onto the DNA and remains constrained close to nuclear periphery. Finally, in G2, mitosis and cytokinesis, Orc1/Cdc6 and PCNA are dispersed throughout the nuclear space. Based on these findings, we propose that DNA replication in T. cruzi is accomplished by the organization of functional machineries in a spatial-temporal manner.