(-)-Epigallocatechin-3-gallate protects spiral ganglion neurons against amikacin-induced apoptosis

摘要

Morphology of spiral ganglion neurons (SGNs) in Sprague-Dawley rats before and after amikacin treatment was observed by transmission electron microscopy. Amikacin induced cochlear SGN apoptosis. Immunohistochemical staining and RT-PCR revealed a decrease in Bcl-2 protein expression, and an increase in Bax protein, caspase-3 protein and caspase-6 mRNA expression following amikacin treatment. (-)-Epigallocatechin-(3)-gallate (EGCG) inhibited SGN Bax protein, caspase-3 protein and caspase-6 mRNA expression, and enhanced Bcl-2 protein expression, thereby decreasing SGN apoptosis. Results demonstrated that EGCG can protect SGNs against amikacin-induced injury.