Phytochemical and TLC studies on stem and leaves of the orchid Dendrobium panduratum subsp. villosum Gopalan & A. N. Henry

摘要

The objective of the present study is to report the phytochemistry of the orchid, Dendrobium panduratum subsp. villosum Gopalan & A. N. Henry. Stem and leaves were dried and powdered using the homogenizer. Powdered materials were extracted with distilled water, benzene, ethanol, acetone and petroleum ether separately. Preliminary phytochemical screening of secondary metabolites was carried out by using Brindha et al, method. Phytochemical studies confirmed the presence of steroids, triterpenoids, alkaloids, tannins, phenols and flavonoids. Physico-chemical and fluorescence analysis were carried out using various organic and inorganic solvents. The colour of the extracts was observed both under ordinary and UV light. TLC studies were carried out using Toluene: Ethyl acetate and Chloroform: Methanol as mobile phase for the presence of phenolics and steroids. These studies provide information in respect of their identification, chemical constituents and physicochemical characters which may be used for standardization of herbal drugs of the present era and enrichment of Ayurvedic pharmacopoeia.